524 EXPERIMENTAL RESEARCHES WITH CHICKEN-CHOLERA MICROBES 



Table III., which died in 14h. 21rn., and between 7h. 29m. and 

 9h. 29m., after inoculation, respectively, (quantity for inoculation only 

 l-40th ccm.) ; on the other hand, the fowl and pigeon from Series III., 

 Table IV., which died between 20h. 15m. and 21 h. 40m., and be- 

 tween 14h. Ion), and 20h., after inoculation, respectively, (quantity for 

 inociilation only l-40thccm.). The tame rabbit did not become 

 seriously indisposed. It reacted again through the formation of an 

 abscess at the point of injection, and a higher body-temperature for 

 some time after the operation. 

 Remarks on Body Temperature, &c. : — 



October 10th — At time of inoculation (noon) ... 40*4° C. 



,, ,, 5.55 p.m. ... 4r0° 



10.15 p.m. ... 41-2° 

 October 11th— 11 a.m. ... 40-47° 



3.30 p.m. ... 40-2° 

 10.10 p.m. ... 40-86° 

 On the morning of this day this rabbit's appetite was not so 

 keen as usual. The seat of inoculation inflamed. 

 October 12th— at 3.15 p.m. ... 40-6° 



October.l3th— 1.15 p.m. ... 4005° 



October 18th — A distinct closed abscess, elastic to the touch, pink 

 at surface, and of pear-shape. 



October 1 9th — Abscess still closed, measuring 30 mm. in length 

 (from apex to base), 23 mm. across the widest part, raised about 

 12 mm. above the level of the adjoining portions of the skin of 

 the belly. 



October 22nd — Abscess still closed, but apparently smaller. 



October 26th — Abscess apparently discharging pus through a small 

 hole. By pressing, pus of a thick, tenacious, and inodorous 

 nature was obtained. The microscopical examination of samples 

 of this pus (cover-glass preparations coloured with methylene- 

 blue solution) did not disclose any chicken-cholera bacteria. 



November — Traces of abscess disappearing. 

 1889. 

 (f) May 9th, 12.45 p.m. 



The tame rabbit (which, I may mention here, was from the first to the 

 last treatment, and afterwards, kept in a large enclosed place) was again 

 treated, after an interval of seven months. This time I injected l-24thccm. 

 (§ minim) of fresh heart-blood (from a rabbit dead of " chicken-cholera " 

 after inoculation) under the skin at the back, between the shoulder- 

 blades. Another vigorous wild rabbit was subjected to the same 



