BY DR. OSCAR KATZ. 527 



they did no receive any food, except water, on the morning of the 

 day when (soon after noon) they were to eat the si)ecially prepared 

 food (conf. Footnote, p. 533). The result was quite satisfactory. 



In order to avoid repetitions, I will mention here that all the 

 bioth-caltures, both those to be sterilised and those to be used in 

 their active state — in the latter case plain rabbit-broth only was 

 the nourishing mediura — had been obtained from fresh heart- 

 blood of rabbits, inoculated for that purpose with virulent broth- 

 culture of the microbes. Such blood was tranferred in small 

 quantities by means of a platinum-loop into the culture-tubes 

 which had been warmed before in the water-bath, so that the 

 broth contained in them showed already a temperature of some 

 thirty degrees Centigr. They were then placed in a thermostat, 

 where they remained for about 24 hours at a temperature close on 

 37-7°C., roughly speaking, between 37-5°C. and 38°C. They were 

 then used immediately afterwards. 



The plan of feeding the ten rabbits on sterilised cultures was as 

 follows : — Two of them were to receive three successively inci-eased 

 portions at certain intervals, the next two one more than the first, 

 the third one more than the second, and so on. 



Section I. 

 1889. 



Two rabbits were fed thi-ee times on steadily increased quantities of 

 sterilised culture in peptonised broth (for one) and plain broth (for the 

 other), as follows : — 2ccin., April 16th; 4ccm., April 17th; 6 com., April 

 19th. 



On April 21st, at about 1 p.m., up to which time the two rabbits 

 appeared perfectly normal, they, as well as a vigorous control-rabbit, were 

 given each 1 ccm. of active broth-culture in some bran. The control- 

 rabbit died between 6.30 a.m. and 7.45 a.m., April 23rd, of "chicken- 

 cholera." One of the principal rabbits, namely that previously fed on 

 sterilised peptonised broth-culture, was seen to die at about 7 a.m., April 

 24th, of typical "chicken-cholera," as the subsequent examination proved. 



