528 EXPERIMENTAL RESEARCHES WITH CHICKEN-CHOLERA MICROBES, 



The other rabbit which had been treated previously with sterilised plain 

 broth-culture, being still alive April 27th, was given on that day, 2 ccm. 

 of active broth -culture.* 



It was still lively May 4th, when it was again fed, at about 1 p.m., this 

 time on 3 ccm, of virulent culture. While another fresh, very robust 

 rabbit, fed on 2 ccm. only, succumbed at 3 p.m., May 5th, to the 

 disease, the former survived. 



On May 10th, at about 2 p.m., it received 4 ccm. of active broth- 

 culture ; the same quantity was given to a control-animal which, however, 

 had not finished eating it until 4 p.m. same day. The latter died between 

 2.15 p.m. and 2.35 p.m., May 11th, of "chicken-cholera;" the principal 

 rabbit survived. 



On May 15th, at about 2 p.m., this rabbit, and a control-rabbit, were fed 

 on 6 ccm. of virulent broth-culture. The latter perished of "chicken- 

 cholera" at 12.40 p.m.. May 16th, i.e., about 22| hours afterwards. 

 Neither did the former withstand this time; it died at 10.50 p.m., May 

 16th, i.e., about 33 hours afterwards. On post-mortem examination, the 

 carcass was found to be very stiff as usual ; typical bacteria in preparations 

 of the blood ; but, with the exception of a plearitis and a slight emphysema 

 of the right lung, the organs looked normal. (Weight of the rabbit, 1490 

 grammes). 



Section II. 

 1889. 



Two rabbits were fed four successive times on the following quantities 

 of sterilised culture in peptonised broth and plain broth, respectively : 

 2 ccm., April l6th ; 4 ccm., April 17th; 6 ccm., April 19th; lOccm., 

 April 21st. 



On April 23rd, at 1.15 p.m., they, as well as a control-rabbit, M'ere given 

 1 ccm. of active broth-culture. The latter died at 2.30 p.m., April 24th, of 

 " chicken-cholera ;" of the two former, one previously treated with 

 sterilised plain broth-culture died about a quarter of an hour later, also of 

 ' ' chicken-cholera. " 



The other rabbit being still alive April 30th — it never exhibited any 

 suspicious symptoms— was fed again on that date, at 2 p.m., on 2 ccm. 

 of virulent broth-culture. It was found dead at 6.30 a.m., May 2nd, 

 whereas another fresh rabbit fed at the same time, along with others, on 

 only 1 ccm. of the same culture, was found dead at about 7 a.m., May 1st. 

 Both succumbed to typical " chicken-cholera." 



* As will be seen further below, the two rabbits of Section IV. and a control-rabbit were 

 fed, the same da5% on 1 ccm. of the same culture for each rabbit. Althoug-h this control- 

 rabbit survived this time, and only one of the former died of the disease 22 hours after 

 being fed, the virulence of the employed culture cannot be doubted. 



