BY DR. OSCAR KATZ. 52& 



Section III. 

 1889. 



Two rabbits were led. jive successive times on sterilised cultures either in 

 peptonised, or in plain broth, as follows : 2ccm., April 16th ; 4ccm., April 

 17th ; 6ccm., April 19th ; lOccm., April 21st ; 15ccm., April 23rd. 



On April 25th, at 1.15 p.m., these two rabbits, as well as a control- 

 rabbit, were given 1 ccm. each of virulent broth-culture. One of the two 

 first mentioned, namely that previously fed on sterilised peptonised 

 cultures, died between 1 p.m. and 2 p.m., April 26th; the control-rabbit 

 succumbed considerably later, it being found dead at 6.40 a.m., 29th 

 April, i.e., roughly speakings after 3i days. The cause of death each 

 time was typical "chicken-cholera." 



The rabbit previously treated with sterilised plain broth-culture, being 

 still alive on April 30th, was fed at about 2 p.m. that day, on 2 ccm. of 

 active broth-culture. It survived again, without ofifering any sign of a 

 change in its behaviour, while a control-rabbit, fed on 1 ccm. only, along 

 with others on the same date (see Section V., mentioned also in Section II.), 

 was found dead (from "chicken-cholera") at about 7 a.m.. May 1st. 



On May 4th, at 1 p.m., the above rabbit was given 3 ccm. of active 

 broth-culture. A very robust control-animal which received 2 ccm. of the 

 same culture (as also did two other rabbits treated before), died at 3 p.m.. 

 May 5th, of typical "chicken-cholera." The principal rabbit remained 

 alive and well. 



On May 10th, at about 2 p.m., 4 ccm. of virulent broth-culture were 

 given to it. It survived again without, apparently, the least inconvenience. 

 A control-rabbit, as already mentioned in connection with the rabbit under 

 Section I., of the same date, succumbed abou.t 24 hours afterwards. 



On May 15th, at about 2 p.m., the rabbit received 6 ccm. of active broth- 

 culture. It withstood also this time, without showing any abnormal 

 symptoms. A control-rabbit, as already mentioned under Section I., died 

 about 22^ hours after feeding. 



On May 21st, six days after the last feeding on 6 ccm. of culture, the 

 rabbit was inoculated with a small quantity of heart-blood, derived from a 

 rabbit which had perished about 6 hours since, of typical "chicken-cholera" 

 consequent on inoculation with virulent broth-culture. The quantity, namely 

 l-48th ccm. (^ minim) was injected by means of a pointed glass-tube, under 

 the skin at a spot on the belly. Another fresh rabbit, of the same sex {$) 

 and about the same size, served for control-inoculation. This control-rabbit 

 died at 1.10 a.m.. May 22nd, or 13 hours afterwards; the autopsy as well as 

 the result of the microscopical examination of cover-glass preparations of 

 blood, secured the diagnosis — "chicken-cholera." 

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