530 EXPEKIMENTAL RESEARCHES WITH CHICKEN-CHOLERA MICROBES, 



The principal rabbit remained alive for good, but exhibited the following 

 symptoms : — At the place of inoculation there was formed a rather large 

 abscess which began to discharge pus for some time, and through which a 

 necrotised portion of muscle and skin was eliminated, similar to the process 

 which may be observed in fowls. The rabbit, which had always a good 

 appetite, became somewhat thinner ; when seen again on June 15th, it was 

 as well- conditioned as before the experiment ; the wound was then not 

 quite healed up. When seen on June 29th, the healing was perfect. 



Section IV. 

 1889. 



Two rabbits were fed six successive times on sterilised cultures in pepto- 

 nised broth or in plain broth, respectively, namely : the first five times 

 exactly as under Section ///., and on the same dates ; the sixth time on 

 22 ccm., April 25th. 



On April 27th, at 1 p.m., each of them, and a control-rabbit, were given 

 1 ccm. of virulent broth -culture. The rabbit previously fed on sterilised 

 peptonised cultures, died from typical " chicken-cholera'' at 11 am., April 

 28th, that is 22 hours after feeding. The other rabbit, as well as the 

 control-animal, did not succumb this time. 



On May 4th, at about 1 p.m., both received 2 ccm. of active broth-culture 

 each. A control-rabbit died at 3 p.m.. May 5th (the same already mentioned 

 under Sections I. and ///., May 4th) ; the rabbit previously treated with 

 sterilised plain broth-cultures perished between 8.45 a.m. and 9.30 a.m., 

 May 6th, that is about 44 hours after feeding, whereas the original control- 

 rabbit was found dead at about 6.30 a.m., May 7th, it having died between 

 9 p.m., May 6th, and that time. 



Section V. 

 1889. 



Two rabbits were fed seven successive times, of which the first six were 

 as in Series IV., and the seventh time was on April 27th, when 45 ccm. of 

 sterilised culture, either peptonised or plain, were given. 



On April 30th, at 2 p.m., each of them, as well as a control-rabbit, 

 received 1 ccm. of virulent culture. The last-mentioned rabbit was found 

 dead at about 7 a.m., May 1st (as already notified under Sections II. and 

 ///. ). The rabbit formerly treated with sterilised peptonised cultures died 

 between 10 a.m. and 11.15 a.m.. May 3rd, or somewhat less than 3 days after 

 feeding; cause of death, typical "chicken-cholera." The other rabbit, 

 treated with sterilised plain broth-culture, did not become atfected. 



On May 4th, at about 1 p.m., it was fed on 2 ccm. of active culture. It 

 died at 4.30 p.m.. May 5th. At the post-mortem examination everything 



