544 EXPERIMENTAL RESEARCHES WITH CHICKEN-CHOLERA MICROBES, 



burrows, * during the time. In every instance it was proved that death 

 was not owing to " chicken-cholera." 



This unfortunate mortality in both the one and the other 

 division, from causes different from "chicken-cholera," was, in 

 my opinion, favoured to a large extent by the extremely oppressive 

 atmosphere and the excessive heat experienced now and then 

 during the course of the experiment.! In the appended Table II. 

 may be seen records of temperature and general remarks on the 

 weather for that period. In quite a number of rabbits the liver 

 and the intestines were diseased, such an appearance resembling 

 that noticed in wild rabbits which were partly starved, or were 

 feeding on unsuitable food. In other rabbits, again, the lungs were 

 pneumonic. So that, after all, it is to be regretted that in this 

 second large experiment, the rabbits used were not all of them in 

 a healthy condition either. 



Ad III. 



In five single experiments fresh rabbits were placed in boxes or hutches in 



which rabbits had died from "chicken-cholera" ; these rabbits, however, 

 . were removed shortly after being found dead. The result was in each 



instance a negative one, inasmuch as a transmission of the disease, in 



those cases^ was not observed. 



1888. 

 (a) August 29th, 9.30 a.m. 



A rabbit was placed in a box, X in which two rabbits, inoculated with 

 a portion of virulent broth-culture, were found dead, one at 7.45 p.m., 

 August 28th, the other at 7 a.m., August 29th. In the box there 



*The burrows in the control-division were alwaj'S opened on the same daj'S, on which those 

 in the disease-division were examined. 



t Most of them (twenty-three) died within eleven days from the beginning 

 of the experiment ; of these, six were dead on one day (Feb. 14th), and nine 

 on another day (Feb. 23rd). 



X The boxes or hutches alluded to in these experiments, were placed in the large en- 

 closure on the Island in such a way that their insides were almost completely sheltered 

 from the rays of the sun. For temperatures [and weather during the experiments noted 

 here, see p. 537. 



