562 EXPERIMENTAL RESEARCHES WITH CHICKEN-CHOLERA MICROBSE, 



Results : 



December 4th.— Owe found dead at 9.30 a.m. (alive at 8.15 a.m.). 

 P.M., Positive. (Carcass stiff; blood coagulated, and of a tarry- 

 appearance ; hypersemia of intestines ; contents of small intestine 

 consisting of slimy, yellowish masses, stained here and there with 

 extravasated blood ; spleen apparently enlarged, cherry - brown. 

 Immense numbers of bacteria of chicken-cholera in the blood. A 

 healthy rabbit inoculated with a small quantity of such blood, 

 succumbed promptly. Cultures derived from blood of this rabbit 

 were further tested, so that with regard to this crow there cannot be 

 any doubt as to the cause of its death). 

 December 9th. — One found dead at 8.30 a.m. P.M., Positive. 

 The three remaining crows, among them the previously inoculated 

 one, were still alive on 



(iv) December 14th, when, at noon, they were inoculated with fresh virulent 

 blood derived from the liver of a rabbit that died of "chicken- 

 cholera " on inoculation. Of these three crows, two, of which one had 

 been inoculated before, received 1 minim each of the blood ; the third 

 ^ minim. 



Jtesults : 



All three crows remained alive and well, thus showing that they 

 were altogether refractory to this treatment. 



1889. 

 ,(v) April 8th, about noon. 



After a lapse of nearly four months, the three crows were inoculated 

 again, at the above time, with liver-blood from a rabbit recently dead 

 from "chicken-cholera"; each crow received the rather large dose of 

 ^ ccm. (4 minims) of such blood injected under the skin of the left side 

 of the thorax. 



In the evening of the same day, and at noon of the following day, 

 they were seen to have eaten only portion of the meat given to them. 



Pesults : 



One crow which some time ago had lost one of its feet through 

 injury, died between 3 p.m. and 3.15 p.m., April 9th; 27 hours 

 after inoculation. The carcass was found resting on a perch, and its 

 head leaning against the wall. 



A second crow which looked dull, and ruffled in plumage, in the 

 evening of April 9th, was found dead at 6.25 a.m., April 10th ; it 

 was lying on tlie floor of the stall. 



