566 EXPERIMENTAL RESEARCHES WITH CHICKEN-CHOLERA MICROBES, 



1888. 

 (i) November 9th, 10 a.m. 



Two rabbits, one quite full-grown, the other nearly full-grown, were 

 given cabbage-leaves sprinkled with 3 ccm. of an active broth-culture 

 for each rabbit. When seen at 12.15 p.m., they had finished their 

 portions of infected food. 

 At 12.30 p.m. they were let go in the aviary. 



Results : 



One observed to die at 10 a.m., November 10th ; the other about two 

 hours later, at 12.7 p.m., both under " chicken-cholera " symptoms. 

 Their carcasses also showed the typical stiffness. A control- rabbit 

 which was found dead at 7.30 a.m., November 10th, was, on examina- 

 tion, proved to have succumbed to " chicken-cholera." 



(ii) November 22nd, 11 a.m. 



Three full-grown rabbits, having besides others arrived on the Island on 

 the previous day, were given cabbage-leaves with 2 ccm. of a fresh 

 broth-culture for each rabbit (at the same time six other rabbits were 

 similarly fed, see p. 538). The three rabbits which were very slow in 

 eating, although they had been left without food for some time, were 

 placed in the aviary at 7 p.m. 



Results : 



One rabbit found dead at 7 a.m., November 23rd. As check for its 

 having died from " chicken- cholera " may be taken a rabbit which, 

 being among the six mentioned (turned into the main enclosure), was 

 also found dead at 7 a.m., November 23rd ; a sample of liver derived 

 from this rabbit, contained the typical chicken-cholera bacteria. 

 The tioo other rabbits being still alive, November 27th, were taken 

 out of the aviary that day. 



(iii) November 23th, 11 a.m. 



Three full-grown rabbits were given green barley-leaves sprinkled with 

 2 ccm. of a fresh broth-culture for each rabbit. Two of them were 

 seen to have eaten their portions of infected food at noon, the third at 

 1 p.m. At 3.15 p.m. they were transferred to the aviary. 

 Results : 



One found dead at 7-30 a.m., November 29th. 



Another found dead at 6 p.m., December 1st ; seen alive an hour 

 before. 



In these two cases a sample of liver was derived, as described 

 pp. 538, 539. The microscopical examination yielded large numbers of 

 typical bacteria. 



The third being still alive, December 14th, was removed from the 

 aviary. 

 Thus five rabbits died in the aviary from " chicken-cholera," 

 two November 10th, one November 23rd, two November 29th 



