570 EXPERIMENTAL RESEARCHES WITH CHICKEN-CHOLERA MICROBES, 



containing five well-nourished guinea-pigs, namely : three young ones^ 

 about i-year old ; one (doe) not quite full-grown, about J-year old , one 

 (doe) quite full-grown. They all had been kept hungry for a while. 

 At the same time, or rather a little before, a fresh rabbit was given 

 4 com. of the same broth-culture ; this rabbit which served in particular 

 as control to two previously-treated rabbits, died about 24 hours after 

 feeding (see pp. 528,529). 

 Results : 



One guinea-pig, the ^-year old doe, died at 10.45 a.m., May 12th, the 

 symptoms shortly before death being similar to those noticeable in 

 '' chicken-cholera " rabbits. 



Another, the full-grown doe, died at 3 30 p.m., May 12th, in pretty 

 much the same way as the preceding one. 



The three i-year old guinea-pigs remained alive, somewhat to my sur- 

 prise. They were watched for weeks afterwards, but were never seen 

 to show any signs of illness. It should be mentioned that they were 

 observed eating the infected food just as well as the two others 

 which subsequently died, and that they must have partaken of it 

 in proportion. 



At the post-mortem examination of the two guinea-pigs, it was first 

 noticed that rigor mortis was very well marked. On removing the 

 skin at the belly, the veins were seen to be gorged with blood. 

 There was a severe peritonitis and pleuritis, especially in the old 

 guinea-pig. Heart distended with blood, which was of a blackish 

 colour. Lungs very voluminous, reddish-white, here and there 

 intersected with darker spots ; on section frothy, crepitating. 

 Spleen enlarged, of apparently usual colour. Stomach filled with 

 food. Intestinal canal very strongly hypersemic ; in one case (full- 

 grown female) the small intestine at different places containing 

 blood-stained liquid masses ; in the other (younger female) the 

 whole of the small intestine .showing externally a dark cherry-red 

 colour, and on being cut open, showing the contents consisting of 

 liquid material very rich in blood. Something similar to such a 

 degree of extravasation of blood into the intestines, I have occasion- 

 ally met with in birds dead from chicken-cholera. The rectum 

 contained solid, although soft, greenish ffeces. 



Cover-glass preparations of blood from heart and liver showed 

 moderate numbers of " chicken-cholera " bacteria ; these were, on 

 the other hand, exceedingly abundant in sap from cut-surfaces 

 of the lungs. 



A stick-culture, derived from heart-blood, in ordinary nutrient 

 gelatine, was in its appearance exactly like others obtained from 

 blood of rabbits or birds which died from " chicken-cholera." 



