574 EXPERIMENTAL RESEARCHES WITH CHICKEN-CHOLERA MICROBES, 



were, in each case deposited in small pouches produced under the 

 skin of the rabbits on the left side of the belly. 



I think it necessary to say that every detail of the experiments 

 was managed under due precautions. 



1888. Series I. 



Silk-threads saturated, November 28th, 11 a.m., with fresh liver-blood 

 (containing large numbers of bacteria), and others saturated with fresh 

 broth-culture of the microbes (this culture had been in the thermostat for 

 a day at 39-39|° C, and for another day in the room at a temperature up 

 to 25° C), were placed on a piece of sterilised brass-wire-gauze in a desic- 

 cator over chloride of calcium. This desiccator was placed, immediately 

 after the threads were put in, in the cupboard of a room where the 

 temperature kept pretty even. 



The virulence of the material employed (blood and broth-culture) was 

 controlled by means of inoculation of a silk -thread impregnated with either 

 blood or culture into a rabbit each. Both rabbits died promptly of 

 *' chicken-cholera," ten and twelve hours, respectively, after inoculation. 



Silk- threads were taken out of the desiccator and inoculated into rabbits 

 after 2, 4, 6, 8, 10, 12, 24, 36, 48, 72, 96, 120, 144 hours from the beginning 

 of the experiment. 



Within this period of six days, from November 20th to December 5th, 

 the temperature near where the desiccator stood, fluctuated between 21|° C. 

 and 18° C. 



Details about temperatures are given in the following table : — 



