678 EXPERIMENTAL RESEARCHES WITH CHICKEN-CHOLERA MICROBES, 



12.15 p.m., 38*^ C. — About five minutes sunshine. 

 12.45 p.m., SQ'^ C — About five minutes sunshine. 



1,10 p.m., 391*^ C, — A few minutes sunshine. 



1.45 p.m., 42 j° C. — About fifteen minutes sunshine. 



2.15 p.m., 38^° C — About twenty minutes sunshine. 



2.45 p.m., 36|° C. — Two or three minutes sunshine. 



3.15 p.m., SS^'' C— A little sunshine. 



3.45 p.m., 33J° C— Very cloudy. 



4.15 p.m., 29^^ C— Very cloudy. 



4.45 p.m., 30 J° C. — Sunshine for about twenty minutes. 



P* ■' ■ [ Sun completely obscured. 



5.45 p.m., 26° C— ) 



From this table there may be seen that the sun was often prevented from 

 making his appearance, by clouds passing by. The day was free from rain, 

 the air was dry, and a southerly breeze was blowing during the time of the 

 experiment. 



The result was : the blood exposed to desiccation in this manaor 

 proved infectious after one, two, six, and eight hours' exposure 

 (when the experiment was terminated). The rabbits inoculated 

 succumbed to " chicken-cholera " in^ respectively, twenty-one, 

 twenty, between thirty and forty, and twenty-eight hours after 

 inoculation. But strange to say, the silk-thread inoculated after 

 four hours' exposure proved ineflicacious in so far as the rabbit 

 was still alive, December 17th, ten days after inoculation. ]t 

 died at about 5.30 p.m., December 18th. Post-mortem exami- 

 nation negative with regard to " chicken-cholera." 



On the other hand, the silk-threads steeped in broth-culture, 

 exposed in exactly the same * way, soon lost their efiScacy. It was 

 only the first time, after one hour's exposure of the silk-threads, 

 that the inoculation of such a thread proved fatal to a rabbit. It 

 died of undoubted '^ chicken-cholera " between fifty-nine and 

 sixty-nine hours after inoculation. In all the remaining cases, two 

 and more hours after the beginning of the experiment, the rabbits 

 did not become infected. 



