580 EXPERIMENTAL RESEARCHES WITH CHICKEN-CHOLERA MICROBES, 



the organisms in their full virulence, was allowed to putrefy at a 

 moderate temperature. From the obtained results it follows 

 that in putrefying or putrid blood of the above kind, they may be 

 found still efficacious after weeks. 



1888. 

 At the examination, on the 2nd September, of a rabbit about four 

 days after its death from " chicken-cholera," in one of the burrows in 

 the large enclosure on the Island, the coagulated blood of the right 

 ventricle of the heart was removed and placed in a small clean, not 

 sterilised, glass-flask which was stoppered and put aside in the 

 laboratory. On microscopical examination on the date mentioned, 

 only the microbes of chicken-cholera were present. 



(i) September 3rd, 11.30 a.m. 



A half-grown rabbit, inoculated with a small platinum-loop full of this 

 blood (not yet putrid), was found dead at 7 a.m. on the 3rd September. 

 P.M., Positive. 



(ii) September 10th, 5 p.m. 



A rabbit, inoculated with about the] same quantity of the blood (now 

 putrid), was found dead at 8.25 a.m. on the 11th September, having 

 died between 7. 15 a.m. and that time {i.e., between 14^ and 15| hours 

 after being inoculated). P.M., Positive. 



(iii) September 17th, 2.10 p.m. 



A rabbit, inoculated with about the same quantity of the blood 

 (putrid), was found dead at 7.40 a.m. on the 18th September. P.M., 

 Positive. 



(iv) September 20th, 10.40 a.m. 



A rabbit, inocidated with about the same quantity of the blood (putrid), 

 was found dead at 5 p.m. on the 22nd September, having died 

 between 1.50 p.m. and that time {i.e., between 51 and 54 hours after 

 inoculation). P.M., Positive. 



(v) September 24th, 11.10 a.m. 



A rabbit, inoculated with about the same quantity of blood, remained 

 alive after this treatment. 



