582 EXPERIMENTAL RESEARCHES WITH CHICKEN-CHOLERA MICROBES, 



mersion objective, as usual, but there was neither a sign of chicken- 

 cholera bacteria nor of any others. By that, however, it cannot 

 be asserted that the blood of the foetus must have been absolutely 

 free from such bacteria, because culture-experiments, which would 

 have been decisive, were not carried out. 



In rabbits also, the results obtained from a few similar micro- 

 scopical examinations were negative. Examined were (1) heart- 

 blood of two out of seven fully-developed foetuses which had been 

 dropped by a doe dead from inoculated " chicken-cholera." In 

 this case, however, the young ones might have been born soon after 

 the inoculation of the mother-rabbit took place. (2) liver-sub- 

 stance of two of several foetuses contained in the uterus of a doe 

 dead after inoculation ; this doe was in the beginning of gestation. 

 (3) liver-substance of one of a few foetuses taken from the uterus 

 of a doe dead after inoculation ; this doe was in about the end of 

 the second week of gestation. 



These negative findings, I confess, cannot claim an absolute 

 value from want, again, of any culture-experiments in gelatine 

 being carried out with samples of the foetal organs ; vet they are 

 quite in agreement with the fact that " chicken-cholera " in rabbits, 

 at least in those with which I had to do, presented itself as a 

 rapidly killing septicaemia, in which, if we except the lungs, any 

 visible lesions of the blood-vessels are rarely found. 



APPENDIX II. 



Remarks on Gamaleia's article " A Contribution to the 

 Etiology of Chicken-cholera, with Notes on the 

 Question of Protective Vaccination."* 



In this article Garaaleia states as tlie result of direct experiments, 

 which he describes, that microbes of chicken-cholera constantly 



* Zur Aetiologie der Hilhnercholera. Nebst einigen Bemerkungen iiber 

 die Schutzimpfungsfrage. Von Dr. N. Gamaleia, Vicedirector der bakterio- 

 logischen Station in Odessa, Centralhlatt fiir Bakteriologie und Parasiten- 

 kunde. Band IV., 1888, pp. 161-168. 



