584 EXPERIMENTAL RESEARCHES WITH CHICKEN-CHOLERA MICROBES 



1888. 



(1) September 27th, 3.50 p.m. 



A healthy pigeon* was killed by chloroform-narcosis. The contents 

 of the small and large intestines, and part of the contents of the 

 stomach (the latter containing green food) were taken under anti- 

 septic precautions, placed together in a test-tube, and mixed and 

 shaken with about 10 ccm. of sterile distinctly alkaline rabbit-broth. 

 This tube was for a while put in a water-bath at 37"^ C. 



Of this mixture, 1 ccm. was injected subcutaneously into each of two 

 rabbits by means of a sterilised pointed glass-tube. 



Besults : 



(a) One rabbit was found dead at 6 p.m., September 28th. P.M , 

 Negative. 



A healthy pigeon, inoculated at 10.30 a.m., September 29th, with 

 a platinum-loop full of heart-blood from this rabbit, remained 

 alive and well. A half-grown rabbit, inoculated at 10.45 a.m., 

 same date, with one platinum-loop full, and a full-grown rabbit, 

 inoculated at 11.30 a.m., same day, with five platinum-loops full 

 of heart-blood of the same rabbit, were both alive at 4 p.m., 

 October 8th, when they were removed from their hutch. 



(b) The other was still alive at 4 p.m., October 8th, when it was 

 turned loose among others. 



(2) October 3rd, 10.40 a.m. 



A healthy pigeon was killed by chloroform-narcosis. The contents of 

 the intestines were collected under antiseptic pi-ecautions, mixed and 

 shaken with sterile rabbit-broth (as above) in a test-tube, and warmed 

 as before. 



Of this mixture injections were made (analogously to the first experi- 

 ment) into two rabbits. At 11.10 a.m., 

 A half -grown rabbit received | ccm. of the mixture, 

 A full-grown rabbit received 1 ccm. of the mixture. 



Results : 



Both of these rabbits were alive at 9 a.m., October 11th, when they 

 were removed from their hutch. 



* All the pig-eons mentioned here were among a consignment of twelve purchased at the 

 Sydney Markets. 



