environs and the cell separates. An activiation of spermatozoa making possible 

 their transition into the genital duct takes place only after this process is 

 finished. Accordingly, there is no passiv piercing, of the cyst cell by inactivated 

 spermatozoa. After the separation the cyst cells having an active nucleus 

 increase rapidly their volume during the transit through the genital duct. 

 The cells take advantage of the secrete sent out of the genital duct wall filling 

 up the genital duct before the departure of testes products. The cell develop- 

 ment during the transit through the genital duct is evident from the nucleo- 

 plasmatic index amounting to 0,51 in the upper part and 0,083 in the lower 

 part of the genital duct. The cells in the lower part of vesiculae séminales 

 have a pycnotic nucleus and represent a reservoir of glycogen and fat drops. 

 During the whole stay of the beetle in the soil the cell organisation remains 

 unchanged; accordingly, no specific activity of the cells is supposed to take 

 place in this period. The cells resume their function till in the spermatophore, 

 they are digested with, transmitting the nutritive materials to the female. 



3. Accessory glands 



Accessory glands of the cockchafer are situated in pairs. They consist 

 of the proper distended part and of a 45 — 55 mm long flagellum. Their secretion 

 consists of 3 components perfectly differentiatable with Masson's trichrom. 

 The basic substance of the secrete is granulous and stains red, the second one 

 is viscous hyaline and stains green, the third basophilic substance stains 

 blue-violet. (The substances correspond to those determined by Anderson 

 in Popilia jajponica Newm.) The substances are not secreted by different zones 

 of accessory glands as Anderson affirms, but simultaneously in one single 

 zone. The secretory zone extends from the lower end of accessory glands to the 

 upper one and further into the flagellum going approximately in the middle of 

 the flagellum during the feeding period. 



Parts of the gland occupied by the zone become a reservoir of the secrete. 

 Glandulary cells take on a paving-tile shape. The secrete of accessory glands 

 and that of flagella are identical. The secreted substance appears at first in large 

 drops, later becomes homogenized. The filling-up of accessory glands and 

 vesicles continues on the whole uniformly during the whole winter being 

 accelerated only in autumn and before emergence of the beetle. The develop- 

 ment of male reproductive organs is in direct relation to the fat body. Its 

 quantitative reduction can be determined on the settled scale. (The scale 

 is based on the experience that the fat cushions disappear first from the trache- 

 al sacs in the thorax, then from stems and finally from the visceral tracheal 

 branches.) The fat body changes qualitatively, too, through the rapid decrease 

 of number of the fat-protein drops. Every disturbance of the relation between 

 the development of reproductive organs and that of the fat body manifests 



112 



