TREHALOSE IN THE DEVELOPMENT OF CELERIO EUPHORBIAE L. 



Трегалоза и развитие молочайного оражнпка 



I. MOCHNACKA and С. PETRYSZYN 



(Institute of Biochemistry and Biophysics, Academy of Sciences, Warszawa, Poland) 



In our studies on Celerio euphorbiae L. we have found the presence of tre- 

 halose in haemolymph and tissues of pupae, луЬ11е we have failed to show 

 it in the caterpillars heamolymph. Thus Ave have undertaken some regular 

 quantitative studies on the occurence of trehalose during the development 

 of this species. 



Caterpillars, pupae and adult moths of Celerio eitphorbiae L. were used. 

 The determination were done separately in haemol3aiiph and in the body 

 after bleeding. 



Identification of trehalose was made by paper chromatographic separation, 

 elution, and estimation of reduction in the water eluate before and after 

 acid hydrolysis. Spots of glucose and trehalose were found. The eluates 

 of trehalose spots did not reveal any reduction before, but did so after acid 

 hydrolysis. 



For estimation of trehalose haemolymph and homogenate of the residue 

 of the body was dialysed against distilled water for 24 hr. at room temperature. 

 Reducing dialisable compounds were estimated in external fluids directly 

 and after 90 minutes acid hydrolysis. The results expressed as glucose were cal- 

 culated for 100 grams of the dialysed tissue. Values found directly were 



Table 1 



Trehalose in the haemolymph and in the remaining body 

 of Celerio euphorbiae (Average values expressed in mg. per. 100 g.) 



185 



