substracted from values obtainned after hydrolysis, the difference giving con- 

 tents of trehalose. 



There is no trehalose in blood or in tissues in feeding caterpillars, nor could 

 trehalose be demonstrated chromatography cally in this stage. The picture 

 is rapidly changed when the adult caterpillar ceases to feed and starts "run- 

 ning". The trehalose content in haeniolymph on the first day of running 

 is about 500 mg %, and reach as up over 1000 mg % on the second day. 

 No trehalose is found in bleeded tissues at this stage. The further period 

 of pupating called "spindle-form" provides the similar data. 



The level of trehalose in haemolymph of pupae remains still about 1000 mg% 

 since pupating till the end of diapausis. During diapausis trehalose is present 

 in bleeded tissues too, in contrary to what is found during the first week 

 of pupal life. It appears there at the end of the first month, reaches a concen- 

 tration of about 300 mg% and disappears at the end of diapausis. 



In the blood of pupae in full development the trehalose level decreases doлvn 

 to about 200 mg%, while it disappears completel}^ from tissues. There is no 

 trehalose in pupae just before eclosion nor in the adult moth. 



The general opinion that trehalose is the main sugar of haemolymph 

 especially at larval stage does not hold for Celerio euphorbiae L. Trehalose does 

 not occure in feeding caterpillars, while its appearance can be regarded as a 

 biochemical mark of the inset of pupating. The presence of trehalose is also 

 typical for the next period of pupal quiescence, whereas its decrease is characte- 

 ristic for the inset of imaginai development in the pupa. Pupa just before 

 emerging of moth as well as imago it self does not contain any trehalose at all. 

 In tissues trehalose is present only during pupal diapausis what seems to be 

 a typical feature for this stage. 



РЕЗЮМЕ 



Авторы провели определение трегалозы у гусениц, куколок и взрослых бабочек 

 Celerio ewphorbiae L. Трегалоза определялась особенно в гемолимфе и в теле после уда- 

 ления крови. Определение проводилось разделением при помощи бумажной хромато- 

 графии, вымыванием и измерением редукции в водном растворе до и после гидролиза. 

 Трегалоза отсутствует у питающихся гусениц, однако является типичным свойством 

 куколок в период закуклования и куколочкой квиэсценцип. Трегалоза отсутствует 

 в конце куколочного периода и у имаго. 



186 



