PHYSIOLOGICAL CHARACTERISTICS OF AN ARTIFICIAL 

 DUPAUSE OF SILKWORM*) 



Физиологические свойства искусственно вызванной дианаузы у тутового шелкопряда 



V. J. А. NOVÄK 



(Biological Institute of the Czechoslovak Academy of Sciences, Praha, Czechoslovakia) 



SUMMARY 



The first two discoverers of the metamorphosis hormones allready, Kopec (1922) 

 and Wiggles worth (1934) have suggested the analogy between the state resiilting from 

 the removing of the brain component of the moulting hormone, known as activation 

 hormone nowadays and that of the true diapause. The subject of the present paper 

 has been the comparison of such a through the removing of brain artificially induced 

 diapause in the silkworn pupae {Bombyx mori L.) with the true diapause in the pupae 

 of the saturniid moth Eudia pavonia L. The course of the О 2 consumption has been studied 

 by means of the Warburg respirometr in the decapitated pupae 2 — 3 weeks after the 

 operation and has been compared with that of the normal and developing pupae of the 

 same species and the diapausLng pupae of E. pavonia. Further the influence of HCN, 

 of the inj'uring and of the rised and loлvered temperature on the О 2 consumption 

 has been studied. It has been found that the О 2 consumption of the decapitated pupae 

 falls to the level of the minimum of the consumption curve of the developing pupae and 

 that it is maintained on that level. By the reduced sensitivity of their О 2 consumption 

 against the temperature changes approached the experimental pupae to the diapausing 

 controls. The injuring was followed by slightly increased metabolism which has not been 

 observed in the developing controls. The HCN — insensitivity has been distmctly in- 

 creased, even if not to the level of the diapausing pupae. The results and the from them 

 following: results are discussed. 



РЕЗЮ31Е 



Предметом настоящей работы является изученпе состояния после изъятия мозга 

 у куколок тутового шелкопряда с пскусственноп диапаузой и ее сравпонпе со свойства- 

 ми нормальной дианаузы у куколок Bombyx mori L. Было обнаружено, что потребление 

 кислорода падает на величину минимума консумачной кривой развивающихся куколок 

 и при этой величине остается постоянным. Снижается также чувствительность потребле- 

 ния кислорода к температуре; тем самым опытные куколки приближались к диапаузи- 

 рующим. На ранения куко^ткп с отсутствием механизма активирующего гормона реагиро- 

 вали малым повышенпем метаболизма, которое не наблюдалось у контрольных куколок. 

 Выносливость против HCN была повьииена, хотя и не достигла уровня диапаузпрующих 

 куколок. 



*) The detailed study will be published later. 



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