THE AMPHIBIA OF OKLAHOMA 



specimens. Oocytes 0.6 to 0.8 mm. ranging up to 1.04. In marked contrast 

 to preceding months, only a few young oocytes seen. 



September — No general condition evident. Often macroscopic indica- 

 tion of preparedness for ovulation is belied by many collapsed follicles, yolk 

 degeneration and little indication of growth of young oocytes. The volumetric 

 data seem to indicate ovaries prepared for ovulation but cytological evidence 

 tends to refute this. Range of ovarian percentages not as great as might be 

 expected. 



Bujo w. woodhousii Girard 



March — Ovary microscopically similar to condition in B. cognatus at 

 same period but volumes dififerent (Tables I and II). No evidence of prior 

 ovulation, oocytes generally undeveloped (diameters mostly about 0.6 mm., 

 largest 0.72). The five animals available are not sufficient to evaluate the 

 statement of Bragg (1940b) that breeding activity begins in March, though 

 such as it is, the evidence is against this expectation. 



April — Ovarian volume suggests (as in B. cognatus) a seasonal peak 

 here of general preparedness for ovulation. Pigmentation intense, oocytes 

 mostly crowded into ovarian cavity (a few in coelom in some individuals). 

 In the eight ovaries observed, oocyte diameter was about 0.9 mm., the larg- 

 est measured being 1.01. Again too few individuals were available to prove 

 conclusively an essential similarity in the ovarian oocytes of the two species. 



May — Great variation in ovarian conditions the rule, though most ovaries 

 show tendencies toward maturity. Collapsed lobes with eggs in coelom fre- 

 quent in those captured in breeding congresses; no evidence of ovulation, 

 past or pending, in others. Many oocytes present in ovarian wall as well as 

 in cavity in eight examined microscopically. No predominating oocyte diam- 

 eter (larger cells usually 0.8 or 0.9 mm., the largest, 1.10; in collapsed ovaries, 

 0.4 mm. appears to be near the median condition). The range of ovarian 

 percentages (Table II) for May is in keeping with these findings. 



]une — Essentially similar to May; an oocyte diameter of 0.5 mm. was 

 characteristic for some animals whereas 0.8 or 0.9 mm. is a better figure for 

 others. The largest oocyte found in this species was present in this series 

 (1.18 mm.). 



July — Conditions essentially unchanged from May and June; oocyte 

 diameter usually 0.5 to 0.9 mm., largest 1.06 mm.; ovarian percentages (22.40 

 for June as contrasted with 16.72 for July) might indicate that most speci- 

 mens had ovulated already. The microscopic evidence does not support this, 

 on the whole. The five animals studied microscopically all had ovaries near 

 the peak of oocyte production. 



August — Variability in ovarian conditions still the rule; two groups 

 indicated, (1) those which apparently had ovulated, and (2) those which 

 had not. In the first, a few young oocytes appeared to be growing in and 

 larger oocytes were beginning degeneration, the latter with 0.9 to 1.0 mm. 

 diameters. In the second group, diameters were generally 0.8 mm., the larg- 

 est 0.97 mm. Nine ovaries were studied microscopically. 



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