Hyla squirella 319 



heard very few of these frogs except for a httle after every rain." We have 

 breeding in June, July and August but transforming frogs recorded from June 

 26- July 10 implies early spring or spring breeding is common. In 1923 (p. 34) 

 we held that "From June ist to loth, three species begin (laying). Bufo 

 quercicus. . . . Hyla squirella chooses the bottom for its singly laid eggs as 

 does Hyla gratiosa." In 1924 (p. 378) we give the season June lo-August 21. 

 Doubtless the bulk of laying in the Okefinokee country comes from April to 

 June because of the transforming tadpoles in June and July. 



Temperature and humidity. Actual ovulation or mating in 1922 came once 

 after a 2 inch rain, maxima 87-96°, average 92°, minima 68-72°, average 70°; 

 after 1.42 rain; after 1.60 inch rain, maxima 81-93°, average 89°, minima 

 65-83°, average 72°; after 1.80 inch rain (August 16) maxima 82-91°, average 

 88°, minima 65-74°, average 71°. In combination we have rains i. 42-1. 80 

 inches, maxima 81-96°, averages 78-92°, minima 65-83°, averages 70-72°. 



Egg-laying process. We did not see the egg laying of any of the four pairs 

 we had in captivity. Apparently they lay single eggs. Whether they are of 

 the Hyla crucifer type or Hyla andersonii type or like Hyla gratiosa we cannot 

 say. Whether they remain at the top and strew eggs singly {Hyla andej-sonii) 

 or several at a time, or whether they progress on the bottom like Hyla crucifer 

 and lay single eggs remains to be observed. 



EGGS 



Egg mass. Pope (19 19, p. 96) is one of the only workers to record their 

 eggs. On April 24, 19 18, at Houston, Texas, he "Caught one mated pair and 

 they laid eggs in a jar the next day. The eggs are laid singly, but tend to 

 cling loosely to the bottom of the jar and to each other." 



We have in every instance thus far held that they were singly laid. But 

 on June 22 at Camp Pinckney we found in one of the water pits a surface film. 

 At first we naturally concluded they were Hylafemoralis eggs. Closer exami- 

 nation made of the individual eggs made us question "Are they H. squirella 

 or Hyla versicolor eggs? Later we found Hyla versicolor tadpoles in the pond." 

 Still they may be Hyla squirella. Our observations on ovulation in this 

 species are too few. 



Egg description. Pope (19 19, p. 96) describes the eggs laid in captivity 

 for him as follows: "In color they are light brown above and nearly white 

 underneath. Diameter of egg not quite one mm., vitelline sac about .12 mm. 

 in thickness, jelly outside that .25 mm. thick." 



On the night of July 3-4, 1922, "at Anna's Pond 11:55-12:00 or later we 

 heard the Hyla squirellas. . . . Four pairs we brought home. All laid between 

 1-7:00 a. m., July 4. Eggs smaller than those of Hyla femoralis. Do they 

 lay singly or below the surface? The outer envelope is very close to the 

 inner envelope." We finally concluded (1923, p. 406) they laid single eggs 

 deposited on the bottom. More observations are needed. 



The actual count of the egg complement of one of the four females (above 

 mentioned) which laid its eggs in captivity was 942 . Another egg complement 

 count is 972. 



