Rana grijlio 375 



The film of May 28 probably was laid May 25 when rains were general at 

 St. George, Hilliard and Waycross. For four days before May 28 minima 

 ranged from 60-70°; maxima, 87-97°. Our temperatures were 64-94°. 



On June 8 the fresh eggs were not laid in rainy weather. The tempera- 

 tures for 4 preceding days on the outside of the swamp were minima 56-70°, 

 maxima 84-87°. Our temperatures were 63-82°. 



The next record June 10, 192 1, was preceded for four days by maxima 

 75-90°, by minima 55-70° for outside stations and by our temperatures 63- 

 84°, and no percipitation for 4 or 5 preceding days. 



In 1922 on July 28 found them breeding. For several days before July 

 28 it had rained. For four days before the nearby Georgia and Florida 

 stations had minima 72-76°, maxima 91-96°. These general conditions of 

 72-76° precipitated general choruses. 



In general we expect this species to breed when the air is humid, and nights 

 and general temperature conditions are 63-70°, but the optimum period is 

 when minima are from 68-78° or higher and maxima mount to 85-96°. 



Egg-laying process. No actual observations on the egg-laying process 

 have been made by us or is in print to my knowledge. The film being similar 

 to that of the greenfrog or bullfrog is doubtless laid in the same fashion. 



EGGS 



Attachment. The eggs are usually attached to pickerel weed (Pontederia) 

 stems in midpond, in the cypress pond on the islands or to "bonnets" on the 

 prairies or amongst the maiden cane along the water courses. 



On May 28, 192 1, we found them amongst pickerel weed and lizard's 

 tail or in the outer edge of the Saururus Zone or attached to flag (Iris). On 

 June 10, 192 1, we had to make a detour to the west of Minne Lake (then 

 filled from lumbering) in a cypress bay. Beside the temporary boat trail in a 

 rather open place among the cypress we found a mass amongst saw grass, 

 maiden cane and wampee. 



On July 28, 1922, in a cypress pond outside of the swamp near Station 

 Branch, found a mass hatching near the outer edge of a mass of arrow-leaved 

 species of plant. 



Egg tnass. On June 12, 19 17, at Theodore, Alabama, we were informed 

 that the large southern bullfrogs which we had captured "laid small beadlike 

 eggs in large masses on the surface of the water." 



The masses found May 24, 192 1 were films at the surface of the water. 

 These films are generally one or two eggs thick. One mass was 15x18 inches 

 in diameter; another 12 x 12. All four masses were at least a foot in diameter. 

 The mass the Cox boys found May 21 was also a film. On May 28, 192 1, the 

 first mass was 10 to 12 inches x 18 to 20 inches; the second 15 x 10 inches. On 

 June 10 'Bud Carter brought me in some fresh bullfrog (R. grijlio) eggs. They 

 found them in maiden cane in Billy's Lake. He said the mass was "15 inches 

 across it." On July 28, 1922, found a film about 16 x 16 inches in diameter. 



In 1920 we wrote (p. 26) "The eggs of the southern bullfrog are not known, 

 and we are not very familiar with its breeding habits. In Georgia, Florida 



