BY K. GREIG SMITH. 45 



usually adheres more or less to the cells ; the blues stain but 

 feebly. B}^ using the night-blue method with the scanty 

 growth obtained on ordinary agar the flagella can be easily 

 stained. They are single and terminal. The bacteria are not 

 coloured by the Gram method of staining. Spores were not 

 obtained and are probably never formed. 



Relations to oxygen and teinperature. — It is a strong aerobe^ 

 and grows best at 30° ; at 37° there is no growth. 



Ordinary glucose-gelatine plate, — The colonies develop slowly. 

 Tn 7 days at 22" they are 1 mm. in diameter, and appear as 

 small, raised, viscid drops. When magnified 60-fold they appear 

 round and uniformly granular, like a thin yeast colony ; the deep 

 colonies are like those upon the surface. Tn 20 days the colonies 

 reach a diameter of 4-8 mm,, and look like drops of yellow bees'- 

 wax. The medium shows no sign of liquefaction, but when the 

 colony is scraped or washed off a pit is revealed. 



Ordinary gelatine plate. — The colonies grow as in the presence 

 of glucose, but much more slowly. 



Ordinary gelatine stroke. — The growth is scanty, narrow, flat, 

 and ivory-white in colour. It slowlj^ gravitates to form a 

 yellowish- white mass. The medium under the stroke is depressed. 



Neutral cane-gelatine stroke. — There is formed a characteristic 

 convex, deep yellow stroke, with waxy drops at intervals, and at 

 the base. The gelatine in contact with the culture is slightly 

 liquefied in three weeks. The colour, bloom, and general appear- 

 ance is that of yellow wax, or of a mixture of yellow vaseline and 

 paraffin. The " tear-drop " appearance of the stroke is character- 

 istic. 



Neatral cane-gelatine stab. — The growth forms in the upper 

 portion of the stab only in a filiform manner, and forms a hemi- 

 spherical, deep yellow nail-head. No gas bubbles are produced. 



Ordinary glucose-gelatine stab. — As cane-gelatine, but neither 

 so luxuriant nor so deeply coloured. ISTo gas formation was 

 observed. 



Ordinary gelatine stab. — Filiform growth in upper portion of 

 stab, with small, raised, white, glistening nail-head. 





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