BY R. GREIG SMITH. 235 



Wort^'-gelatine. — The colonies are raised and like drops of whey. 

 When magnified they appear round and uniformly granular. 

 Sometimes the margin is striated as if from the growth flowing 

 down the dome-shaped colony. The deep and subsurface colonies 

 are small and coarsely granular. 



Saccharose-gelatine plate. — The colonies are transparent and 

 hemispherical, like exuded drops of glycerine. 



Nutrient agar plate. — The colonies are round, slightly raised, 

 translucent white and moist glistening. When magnified the}'' 

 appear round with a smooth edge. There are granules around the 

 centre, but otherwise the structure appears homogeneous. The 

 deep colonies are rounded to elliptical, and contain large granules. 



Saccharose-2jepto7ie-agar plate. — The colonies are hemispherical 

 and whitish, like drops of starch paste. When magnified they 

 appear rounded, and have a blistered surface; the margin is 

 apparently smooth. 



Nutrient gelatine stab. — The stab is filiform and white; the 

 nail-head is round, flat, white and glistening. The medium is 

 slowly liquefied; in 14 days at 22^ the liquefied area is slightly 

 funicular, and at the top of the stab the medium has been con- 

 sumed, leaving an air-bubble. 



Glucose-gelatine stab. — As with nutrient gelatine. 



Saccharose-gelatine stab. — A filiform stab with an hemispherical 

 drop of transparent fluid as a nail-head. The fluid increases and 

 flows over the surface of the gelatine. As this occurs, the stab 

 develops lenticular and spherical pockets of almost transparent 

 gum. A liquefaction of the medium below the surface was noted 

 after a month. 



Glucose-gelatine stroke.— The growth is rough and narrow, with 

 an expanded lower portion like the nail-head on nutrient gelatine. 

 The stroke becomes rough and ribbed, dry glistening and whitish. 



Nutrient agar stroke. — A thin translucent white, almost trans- 

 parent, layer is formed. It is always scanty, and the condensed 

 water has a white sediment and no film. 



The wort contained saccharose. 



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