BY R. GREIG SMITH. 407 



the agar surface. At 37° in 24 hours the growth is broad, raised, 

 _^ glistening, with a translucent margin and opaque centre. The 

 culture is apparently gummy. The condensed water has a film. 

 In 48 hours the growth by reflected light appears homogeneous 

 and the colour of the medium. At 22° in 24 hours the growth 

 is broad, whitish, very rough and undulating. The margin is 

 more glistening than the centre. The condensed water carries a 

 film. In 48 hours the growth is mottled and pale buff". At 15° 

 the growth is as at 22°, but less luxuriant. On this medium at 

 22° the growth is very like that of Bad. acacice^ but differs in 

 having a lumpy (gelatinous) consistency, while that of Bact. 

 acacice is homogeneous. At the higher temperature the differ- 

 ences are more pronounced. 



Potato. — A dry, pale buff scattered growth spreads irregularly 

 over the medium. It is raised and becomes undulatino-. A 

 glistening appearance begins at the margin and spreads inwards 

 as the colour deepens to orange-yellow. 



Bouillon. — The medium becomes turbid and forms a strong 



o 



surface ring and a floating puckered film. The indol reaction 

 was obtained, but nitrite is not produced as with Bact. acacice. 

 In nitrate-bouillon the nitrate is reduced to nitrite. 



Milk. — The medium slowly coagulates and the reaction is 

 faintly acid. 



