284 ERNE8T WARREN. 



The formation of such crystals also occurred in eggs laid 

 by a female raaia paii'ed with a male belina, as will be seen 

 in the account given below. 



Maia ? x belina c?. — In one of the pairings a female 

 maia was placed with a captured, wild, fresh-looking male 

 belina (see list, p. 273, d). There is here more evidence 

 that copulation occurred, and that the semen exerted an 

 influence on the eggs of the female maia. The moths were 

 placed together on October 16th, and the female soon began 

 to lay very freely. Two hundred and one eggs were laid, and 

 these were very slow in shrivelling. On November 10th some 

 of these eggs were examined, and on December 3rd, when it 

 was obvious that hatching would not take place, all the 

 remaining eggs were opened. Altogether forty-eight (i.e. 

 about 24 per cent.) showed with a hand-lens an opacity on 

 one side of the yolk. Examination with the microscope 

 proved that a certain amount of segmentation of the yolk 

 had occui'red ; large rounded or polygonal cells crowded 

 with yolk-globules were present. On treatment with weak 

 methyl blue^ a deeply staining blue spot became defined in 

 each area in the course of a day, and there appears little 

 doubt that this was the nucleus. There was not much 

 difference in the condition of the eggs on November 10th 

 and on December 3rd, except that on the latter date drying 

 was taking place and death of the large yolk-cells was 

 occurring. The nucleus had become less defined and was of 

 inflated size and of irregular shape. Portions of the cells had 

 apparently disintegrated into an opaque, white, finely granular 

 substance. In some of the eggs clumps of needle-shaped 

 crystals were found, and they were quite the same as those 

 mentioned above, and occurred among, but apparently not 

 inside, the yolk-containing cells. 



In the case of the second pairing, a female maia was 

 placed with a very shabby, wild male belina. The male soon 

 died. Fairly numerous eggs were laid, but these shrivelled 

 rather quickly and there was no sign of development. 

 Doubtless the male was spent and no copulation took place. 



