454 THE RED STRING OF THE SUGAR-CANE, 



Relation to oxygen. — The bacterium is a facultative anaerobe, 

 there being a slight growth with gas-production under the mica 

 plate in nutrient agar and saccharose-agar cultures. 



Glucose-gelatine colonies. — They are raised, sometimes as hemi- 

 spheres, circular, white and gummy. Microscopically they have 

 granular radial striations from an almost opaque centre to the 

 transparent edge. The deep colonies are opaque and rounded. 



Xutrient gelatine colonies. —These are raised, white, glistening, 

 and circular. Microscopically they are very finely granular with 

 slightly erose margin and turbid or fibrous centre. The deep 

 colonies are irregularly rounded, dark brown and somewhat zonate. 



Nutrient agar colonies. — These are white, slightly raised, and 

 from being round they become amoeboid. Microscopically they 

 are very finely granular. The deep colonies are oval or lenticular 

 and semi-opaque. 



Glucose-gelatine stah. — The, needle track becomes filiform and 

 rough. The nail-head is white, raised, and fiat, with irregular 

 margin. Gas bubbles are produced in the medium. By the 

 14th day the porcelain-white nail-head has become depressed 

 centrally. 



Glucose-gelatine stroke. — The growth is raised and white, with 

 an irregular, erose margin. The colour becomes porcelain-white 

 and the margin lobular. The growth becomes depressed from a 

 slight liquefaction or rather consumption of the medium. 



Nutrient gelatine stab. — More scant}^, but otherwise similar to 

 glucose-gelatine. A slight stratiform liquefaction had taken 

 place by the 30th day. 



Nutrient agar stroke. — X translucent white, broad, raised 

 growth with spreading base is formed. 



Bouillon. — The medium becomes very turbid, with a delicate 

 white film and cohesive white sediment. The indol reaction was 

 obtained and nitrates were reduced to nitrites. 



Milk. — The medium had curdled by the 14th day and carried 

 a slimy film. 



Potato. — The growth is raited, fatty and dirty-white, but 

 deepens to a flesh colour. 



