BY R. GREIG SMITH. 617 



never amoeboid. It appears either moist, glistening, or fatty, 

 and the consistency is soft like that of butter. The condensed 

 water generally has a flocculent yellowish sediment, but never 

 has a film. 



Saccharose-peptone-agar stroke. — With all groups, there is 

 formed a broad convex ridge, undulating as if containing gela- 

 tinous lumps. The growth is translucent and indistinguishable 

 from the medium. The consistency is gelatinous. 



Gelatine plate. — Group a : The colonies grow slowly; on the 

 third day they are white and punctiform. When magnified, the 

 deep colonies are seen to be round, moruloid or like woolly tufts, 

 with outstretching fibres interspersed with cellular clavate pro- 

 cesses. The surface colonies are rounded erose, with a granular 

 centre and clouded margin. On the fourth day the surface 

 colonies appear depressed and are seen, microscopically, to be 

 deepty pitted and wrinkled, and to have a rough margin. On 

 the sixth day the surface colonies consist of a dry, wrinkled film, 

 with a central white point. There is a slight softening of the 

 gelatine, and in crowded plates the medium becomes slimy. 

 Groups /3 and /3/3 : White circular crateriform liquefied areas con- 

 taining a white sediment are formed in two days. Microscopi- 

 cally the sediment consists of coarse granules, and the margin of 

 the colony (liquefied area) is eiliate. The deep colonies appear 

 circular and either opaque or coarsely granular, with a eiliate 

 edge. 



Gelatine stab. — The stab is primarily filiform, with a slight 

 depressed'surface growth. In from four to six days, the needle 

 track is beset with coarse white arborescent outgrowths, and the 

 gelatine at the surface is slightly liquefied and consumed. The 

 liquefied medium is covered with a deeply wrinkled film. Groups 

 /3 and /3/3 : The growth is at first" white and filiform, but the 

 medium is sooner or later (1-2 days) liquefied, the top of the stab 

 becoming funicular ; finally the entire stab becomes saccate. 

 There is formed a thin surface-film. 



Bouillon. — Groups a and /3: The medium becomes faintly turbid 

 and covered with a strong but easily detachable wrinkled white 

 41 



