676 



DETERIORATION OF SUGAR CRYSTALS IN BULK, 



is to say, bubbles of gas were being given off. A film of the 

 solution showed the presence of a yeast, and the gum bacillus of 

 the normal type was isolated by plate culture. 



A moist raw sugar had been tested in the laboratory of the 

 Colonial Sugar Refining Co., and had shown the following percent- 



ages of reducing sugar 

 10-5-01 ... 

 25-5-01 ... 

 25-6-01 ... 



1-43 

 2-90 

 4-46 



From an agar plate infected with the sample, there were 

 obtained 30 colonies of the normal type, 3 of the derived type, 

 and 1 inert bacterium. 



A soft yellow refined sugar which contained 2-5 % reducing 

 sugar, and which had undergone no change during a storage 

 period of 3 J months, contained, for every 12 inert bacteria, 2 of 

 the normal and 1 of the derived type. 



Another similar sugar which contained the same percentage of 

 reducing sugar, and which had shown no further inversion in 

 three months, contained, for every 30 inert bacteria, 15 bacilli of 

 normal type. 



A raw sugar which had been damaged by water in November, 

 1895, showed a very heavy inversion. The water content, after 

 the damage had been done, was 6-97 %. The analyses which 

 were made in the Sugar Company's laboratory from time to time, 

 are as follows. 



Date of Analysis. Eeducing Sugar %. 



Nov. 22, 1895 09 



3-1 

 4-3 

 9-6 

 11-6 

 15-6 

 20-8 

 26-3 



3 



^^{f;; 



dextrose 18-9 

 ulose 13-8 



