BY H. G. CHAPMAN. 



95 



and tested. It Ccaused a rapid secretion, though it had stood in 

 the laboratory at 17° C. in a covered beaker. 



The sterilized extracts keep for several weeks. Active secretion 

 was produced by an extract from a dog made fortj'^-two days 

 previously, and with that of a cat made thirty-seven days l^efore 

 use. The rapid deterioration noted b}^ May^^ has not been 

 observed, though the extracts used have been kept at tempera- 

 tures from 3°-20° C. to test the effect of temperature. May 

 stated that the extracts from the mucosa ceased to contain 

 secretin in two days, even when kept in an ice-chest. The follow- 

 ing table shows some results obtained : — 



This difference from May seems difficult to explain, but in two 

 cases out of over fifty the absence of secretin was noticed. One 

 extract was from a dog prepared seven days before testing, the 

 other from an echidna made twenty-four hours previously. 



JResidts. — As the work of Bayliss and Starling has been 

 abundantly confirmed, there is no need to more than mention that 

 their results with acids and extracts were confirmed. Secretion 

 was also found to be brought about by the injection of pilocarpine 

 into the circulation. This secretion differs from that produced 

 b}^ secretin in that it is abolished by the subsequent injection of 

 atropine. That atropine does not abolish or atTect the secretion 

 called forth by secretin was shown by Camus and Gley,^^ Bayliss 

 and Starling, and Wertheimer and Lepage. ^"^ Atropine inhibits 



* Shot Sept. 15th. Intestine under absolute alcohol until Sept. 27th. 



