BY 11. G. CHAPMAX. 99 



the tubes of series (b) and (o) in from twenty-five minutes to four 

 hours, varying with the quantity of juice. Xo alteration was 

 perceived in the fibrin in tubes of series (a), even after weeks. 

 The sterility of the tubes was tested with agar and gelatine plates. 



Controls were also made with serum from the blood of the dog, 

 from whom the leucocytes were obtained. Xo activation of the 

 juice was observed. 



These results were repeated upon five occasions, four times 

 using sodium fluoride, and once potassium arsenite. 



Protocols of two series are appended : — 



A 10 c.c. 2-0 XaF-0'1 c.c. pancreatice juice- 0"5 c.c. enterokinase solution 



[ — fibrin. 



B ,, - „ — ,, „ 



^ >j ~ J) ~^ >j ft 



D ,, — ,, -f- fibrin. 



E „ - „ - „ 



F „ - „ - „ 



G ,, — ,, —fibrin impregnated with 



rieucocvt-es. 

 H ,, - „ - „ ' 



J „ - 



Ai lOc.c. 2% K3ASO . - 0-25 c.c. pancreatic juice - 0-25 c.c. enterokinase soltn. 



[ — fibrin. 



B^ ,, - „ - 



ri _ _ 



D- ,, — ,, -fibrin. 



T : _ _ 



-^ -5 j; ?! 



Fl - — 



G^ ,, — ,, —fibrin impregnated with 



[lencoeytes. 

 H ,, -7- ,, -f „ 



All the tubes were kept in a water-bath at 39° C. A. B. and 

 C were digested in two hours; G, H, J in three hours; A^, B^, 

 C\, Ct\ H\ and J^ in twenty-five minutes; but D, E, F, D^, E^, 

 and F^ showed no change in seventy-two hours, and D, E, and 

 F none in ten weeks. No tube yielded any culture upon agar 

 and gelatine plates. 



