BY P.. GREIG .SMITH. 173 



BouiUon. — The medium becomes verv turbid, develops a loose 

 film and a coherent sediment. Indol Ls formed and nitrates are 

 reduced to nitrites. 



MUk. — The medium becomes slightly acid, but Is otherwise 

 unaltered. 



Bacillus lini ii., n.sp. (Organism " b."; 



Shape, etc. — The organism appeared as a large rod, staining 

 deeply but irregularly; the outline of the freshly isolated celLs 

 was generally lobular. The dimensions were variable, 1-2- 

 1-5 : 3-10 /t; the a%-erage being 1'5 : 5 ^. In boaillon and saccha- 

 rose media, chains of cells and thread forms were found. The 

 cells were coloured by the Gram method of staining, but a few 

 were decolorised, these being in all probability dead cells. After 

 about nine months' cultivation, the spongy protoplasm of the cell 

 had condensed, the rod stained uniformly and measure<:l 1-2 : 3-6/x. 

 The spores, when mature, were generally oval, measuring 

 1-2: 1-7-2^; occasionally they were reniform. As a rule thev 

 were central, but some were excentric. The microbe was verv 

 feebly motile; the flagella were numerous and peritrichous. 



Relation to temperature, etc. — Sh'me is produced at ordinarv 

 temperatures. There was practically no growth under the 

 anaerobic conditions that prevail under the mica-sheet in plate 

 cultivations. 



Glucose-gelo^tiae plate. — The colonies appeared as circular, white, 

 liquefied areas, and when x-iewed microscopically were brownish- 

 black and granular like a yeast colony. As the liquefaction of 

 the medium proceeded, the colony consisted of a white spot in a 

 crateriform, liquefied area. The subsurface colonies were verv 

 slow to liquefy the gelatine, and appeared flat, white and 

 glistening. 



Nutrvirit agar plate. — The colonies were raised, circular, and of 

 a pale cream colour; the surface was rough, although the sheen 

 was that of a fat. Microscopically, the colour was grey-brown, 

 the centre was opaque and indefinite, the margin clouded and the 

 edge waved, showing the finely granular structure of a yeast 

 colonv. 



