412 KOTES AND EXHIBITS. 



insect-remains. In 1893, while examining the country in the 

 vicinity of the South Bulli (Bellambi) Colliery, I obtained a pair 

 of valves of a crustacean, apparently of the same species as the 

 Newcastle form, in similar strata to thos.e in whicli the Newcastle 

 fossils occur, and associated with Glossojjtaris. The occurrence 

 of Estheria sheds a little additional light on the geological con- 

 ditions obtaining during the deposition of this series of rocks." 



Mr. Kesteven exhibited sections of the cervical cord of a dog,, 

 and mesial, sagittal sections of the brain of Hyfa aurea treated 

 as follows — (1) fixed in absolute alcohol; (2) cleared in cedar oil; 

 (3) embedded in paraffin; (4; cat, and mounted by the water 

 (method; (5)mordanted in MuUer solution at 45°C., 48 to 76 hours; 



(6) stained in 1 % hfematoxylia and 2 % acetic acid, 2-4 hours; 



(7) differentiated in (a) 25% potassium permanganate, (b) 2% 

 oxalic acid -t- 2 % potassium sulphite, equal parts; (8) washed in 

 a weak alkaline solution. Although the staining is a moditied 

 " Weigert-Pall," the results are completely different, axis- 

 cylinders, nerve-cells (tyroid substance), and the nuclei of all 

 cells staining black or brown, all other tissue remaining quite 

 unstained. The advantages of the new stain are that the ditfer- 

 entiation does not take place rapidl}'', so that one may success- 

 fully treat a large series on a slide; and the whole process may 

 be completed in five or six days. Since the fixation is by abso- 

 lute alcohol, the method is limited to small brains or small pieces 

 of tissue, but it should prove extremely useful for studying these. 

 Since the pictures are black on a white background, they are 

 admirably adapted for photographing. 



Mr, T. H. Johnston exhibited a series of Entozoa collected in 

 New South Wales, including Ascaris canis and Dipylidium 

 caninum, both from the cat and the dog; Heterakis ivflexa, from 

 a fowl's intestine; H. papilloma, from the caeca of a fowl, and of a 

 turkey; JI. maculosa, from a pigeon, this being the first record of 

 its occurrence in Australia; S^nroptera sp., forming nodules or 

 "kernels" in the muscles of cattle, the presence of this or a 

 similar parasite having been already recorded by Dr. Cleland 

 from cattle and camels in West Australia; Cceymrus serialis, the 



