BY R. rjREIG-SMITH. 



175 



Nutrient gelatine-stab. — k flat, spreading nail-head, the canal 

 with arborescent outgrowths in the softened gelatine. 



Glucose gelatine-stab. — As with nutrient gelatine, but a stronger 

 growth and gas-bubbles in the medium. 



Glucose gelatine-plate. — Circular, white colonies in a zone of 

 softened medium. Finely granular structure. 



Glucose gelatine-stroke. — A white raised growth softens then 

 liquefies the gelatine. 



Milk.—T\\Q casein is coagulated, with formation of a clear, 

 acid whey. 



Bouillon. — Turbid with film and sediment. Indol is produced, 

 and nitrates are reduced to nitrites. 



Starch-media. — A weak, filtered suspension did not react with 

 iodine after two months; a stronger, unfiltered suspension gave 

 a reddish-violet colour with iodine. 



Potato. — A raised, fat-glistening, ivory-white growth; medium 

 slightly darkened. 



Saccharose-peptone-fiuid. — Opalescent medium with film and 

 sediment. Formation of levan-gum and inversion of saccharose. 



Eucalyptus-manna-agar. — A raised, gummy growth. 



Fermentation of sugars. — Acid and gas from dextrose, saccha- 

 rose and lactose. Glycerine is not attacked. 



While testing the action of the microbe upon the various 

 sugars, I also tested a race of Bac. levaniformans obtained from 

 refined sugar, and of Bac. eucalypti recently isolated from the 

 tissues of an Angophora. 



Dextrose. 



Bac. hf.miphloia' ... 

 Bac. eucalypti 

 Bac. Uvaniformans 



Acid and gas 

 Acid 

 Faintly acid 



Saccharose. 



Acid and gas 

 Acid 

 Faintiv acid 



Lactose. 



Acid and gas 

 No action 

 No action 



Glycerine. 



No action 

 No action 

 No action or 

 faintly acid 



By the numerical system of the American bacteriologists, the 

 indices for these three microbes are — 



Bac. hemiphloicp, n.sp. ... Bac. 2 21-111 3 023. 

 Bac. eucalypti G.-^. ... Bac. 221-2322033. 



Bac. levaniformans G.-S.... Bac. 121-2323022. 



