BY R. GREIG-SMITH. 



731 



The rate of disappearing was determined in one instance, in which 

 0"122 gram was shaken up with 100 c.c. of water. 



Chloroform taken by weight ... ... ... ... 0' 122 gram. 



Chloroform determined at once ... ... 0*114 gram. 



Chloroform determined after 20 hours ... 072 gram. 



The gi'owth of soil-bacteria in chloi"oformed exti-acts of soil was 

 then examined. The exti'acts, after treatment with chloroform- 

 water, were sown with a suspension of bacteria from a soil which 

 had been previously chloroformed and stored in a fairly dry con- 

 dition (moisture = 1:"5%). 



Experiment v. 



Soil-extract containing chloroform 

 in parts per million. 



Non( 



41 

 414 



Growth of mixed soil-bacteria. 



100 



144 



79 



A stimulation of the growth of the soil-bacteria has occurred 

 in the presence of 41 parts of chloroform per million, while 414 

 parts have evidently been too much, as they have acted as a 

 toxin. 



Experiment vi. 



This agrees generally with the previous experiments, although 

 the stimulatiug effect of the larger quantity is not so pronounced 

 as the smaller with the soil -bacteria. Micr. ca7ididus had, in 

 some accidental manner, got into the suspension of Bar. jjro- 

 digioHUS. 



The extracts in Experiments i. to vi. had always been nutritive, 

 as was shown by the bacteria, that grew in the unchloroformed 

 extract, being always greater in number than in the water-control 



