120 REPOKT OF COMMISSIONERS OF INLAND FISHERIES. 



development, but were later re-absorbed by the predominating 

 normal regenerative processes. 



c. Various Methods of Mutilation and Residts. 



The observations just recorded suggested the possibility of getting 

 similar results by artificial mutilation. In one experiment the tips 

 of regenerating JDuds were touched with a redhot forceps. I thought 

 it might be possible to destroy certain cells of the regenerating struc- 

 ture and thus produce an abnormality in these parts. Although six 

 or more buds at different stages of divelopment were operated upon, 

 no positive results were attained. The wound would nearly always 

 heal over and the regenerative processes proceed again in a normal 

 manner. 



A different method of mutilation was then tried by cutting the 

 regenerating buds at various regions. In about six cases the tips 

 of the buds were slit with a sharp instrument; six more specimens 

 were injured by nipping out small pieces from the fundaments of 

 various segments; and again, in other instances, the buds were cut 

 off at different levels. These mutilations were made upon buds 

 at all stages of development, ranging from minute papillse up to 

 stages, in which the segments were clearly evident, but in no 

 instance was an abnormal structure obtained. In all cases the 

 result was either that the whole regenerating bud would be finally 

 dropped at its base, or else the wound would heal and the regenera- 

 tion continue. An illustration of the latter result is shown in Figure 

 22. This regenerating bud had been cut near the tip with a pair of 

 fine scissors. The wound healed rapidly, and the direction of growth 

 among the regenerating cells was such that the scar (s) was finally 

 pushed to one side. After the moult only a slight trace of the wound 

 was evident. 



In a third experiment, mutilations were made by cutting normal 

 limbs at various levels. In all these mutilations of this character, 

 the limbs were either autotomously dropped after the operation, or 

 else regeneration began at" the region of injury. This experiment was 



