2 ARKIV FÖR ZOOLOGI. BAND 13. N:0 25. 



Here, I will remark, that from the begimiing of my studies 

 of the coelomic corpuscles I was fully conscious of my results 

 being based on methods which certainly are sufficient for my 

 j)urpose, but do not answer the requirements of modern re- 

 searches of the structure of the cell-protoplasm and its chemical 

 nature. On account of my superficial knowledge of chemical 

 and physiological methods, I have limited myself to an exa- 

 mination of the coelomic cells from the view alone that they 

 are free organisms in their retrenched world. 



With regard to the methods employed by me, I beg to 

 ref er to my earlier papers. Concerning fixatives, the employr 

 ment of sublimate, of osmic acid, of Carnoy's or Zenker's 

 Solutions was for several reasons impracticable. For my 

 purposes good and hasty fixation was obtained with Peré- 

 NYi's liquid or formaldehyde in concentrated solution. 



Among staining matters Heidenhain's iron-alum haema- 

 toxylin method was found to bring to view exceedingly fine 

 structural details of the plasmodia and their net-works. 



'-- I? My account of the coelomic corpuscles of the holothurians 

 in its present state is based on studies of the following species 

 which are stationary in the neighbourhood of the swedish 

 marine biological station Kristineberg viz. Labidoplax huskii 

 (Mc Intosh), TJiyone fusus (O. F. Muller) Cucumaria lactea 

 (FoRBES and Goodsir), C. liyndmani Thompson, C. elongata 

 DuBEN and Koren, Psolus phantapus (Strussenfelt), Me- 

 sothuria intestinalis (Ascan. and Rathke) and Stichopus 

 tremula (Gunn.). My researches may be looked upon as an 

 attempt to throw light on some problematic or rather unknown 

 questions with regard to the activity of the plasma-amoebocytes 

 and their faculty of producing crystals or spicula, and, besides, 

 to the presence of red blood-corpuscles and of fibrine. Of 

 course, I also paid attention to other forms of leucocytes, 

 though more by the way, for inst. to the »bladder-amoebo- 

 cytes» (= blåsamoebocyter) and to the white and coloured 

 »amoebocytes with spherules» (= kulamoebocyter). Con- 

 cerning these terms, I refer to my earlier papers.^ 



^ Om amoebocyter och a^ndra kroppar i perivisceralhålan hos Echino- 

 dermer. I. Asterias ruhens; II. Parechinus miliaris. — Arkiv för Zoologi (ut- 

 givet af K. Svenska Vetenskapsakademien); Bd 12, N:is 4 och 14, Stock- 



