292 THE POPULAR SCIEXCE MONTHLY. 



Thus, on the 6th of July, at eight o'clock in the evening, sixty hours 

 after he had been bitten on the 4th, in the presence of Drs. Vulpian 

 and Grancber, we inoculated under a crease made in the skin of the 

 hypochondrium of little Meister a half-syringe Pravaz of marrow of a 

 rabbit that had died of rabies on the 21st of June, which had been 

 kept since that time, or for fifteen days, in a flask of dry air. 



New inoculations were made, always in the hypochrondres, under 

 conditions of which a table is here given : 



July 



I thus made the number of inoculations thirteen, and the number 

 of days of treatment ten. I would say, furthermore, that a smaller 

 number of inoculations would have been sufficient. But it is easily 

 conceivable that in tbis first trial I should have acted with particular 

 caution. 



We also inoculated, by trepanning, two new rabbits with each of 

 the several marrows employed, in order to test their states of virulence. 

 The observations on these rabbits permit me to assert that the marrows 

 used on the 6th, 7th, 8th, 9th, and 10th of July were not virulent, for 

 they did not make the rabbits mad. Those of the 11th, 12th, 14th, 

 15th, and IGth of July Avere all virulent, in proportion as the marrow 

 was fresher. Rabies declared itself after seven days of incubation in 

 the rabbits of the 15th and 16th of July ; after eight days in those 

 of the 12th and 14th ; and after fifteen days in those of the lltli 

 of July. 



I had thus in the last days inoculated Joseph Meister with the most 

 virulent virus, that of the dog strengthened by several passages from 

 rabbits to rabbits ; it Avas a virus that gave rabies after seven days 

 of incubation to these animals, after eight or ten days to dogs. I was 

 justified in venturing on this experiment by what had taken place 

 with the fifty dogs of which I have spoken. When the state of im- 

 munity is reached, we can, without inconvenience, inoculate with t])e 

 most virulent virus, and in any quantity ; and it has seemed to me 

 that this had no other effect than further to confirm the condition of 

 refractoriness against rabies. 



