Table 16. Distribution of phosphorus compounds in ram semen 



(Results based on analysis of 16-8 ml. ram semen, representing 14 

 ejaculates from 7 rams; average volume of single ejaculate 1-2 ml.; 

 3,050,000 spermatozoa/yal. Semen separated by centrifugation for 20 min. 

 at 10,000^, into sperm, 26% v/v, and seminal plasma, 74% v/v.) 



mg. P/100 ml. semen 



In whole In sperm In seminal 



semen plasma 



Total phosphorus 328-5 186-7 141-8 



Acid-soluble phosphorus (in trichloro- 

 acetic acid extract) 



Pjnorg (orthophosphate determined as 



MgNH4P04) 10-3 3-1 7-2 



Po (phosphate reacting directly with 

 molybdate) 11-9 4-7 7-2 



P7 (phosphorus which appears as ortho- 

 phosphate after 7 min. hydrolysis 

 withN-HCl) 15-4 8-1 7-3 



P30 (phosphorus which appears as 

 orthophosphate after 30 min. hydro- 

 lysis with N-HCl) 15-9 8-6 7-3 



Ptot. ac. sol. (total acid-soluble phosphate 



determined after incineration) 159-4 27-4 132-0 



Patp (labile phosphate of adeno- 

 sine triphosphate determined in the 

 Ba-salt by the method of Parnas 

 and Lutwak-Mann, 1935) 4-3 4-3 00 



Phexose (phosphate of 6-phosphohexose 

 determined in the supernatant from 

 Ba-ATP) 6-2 60 0-2 



Phospholipid phosphorus (extracted with 

 ethanol and ether from the residue 

 insoluble in trichloroacetic acid) 30-8 27-9 2-9 



Deoxyribonucleic acid-phosphorus (ex- 

 tracted with KOH from the residue 

 left after removal of acid-soluble P 

 and phospholipid; precipitated from 

 the KOH extract by acid) 1110 1110* 00 



Residual phosphorus (left after removal 

 of acid-soluble compounds, phos- 

 pholipids and deoxyribonucleic acid) 27-3 20-4 6-9 



* Corresponding to 3-2 x 10~^ mg. deoxyribonucleic acid/sperm cell. 



107 



