Adrenaline and Noradrenaline 181 



More recently, Wajzer and Brochart (1947) reported on the isola- 

 tion from boar sperm of a barium-precipitable fraction containing a 

 mixture of two phosphagens, phosphocreatine and phosphoarginine. 

 The distribution of the two phosphagens in the gonads and in 

 sperm remains open to further investigations. An important contri- 

 bution in this field was made by Green wald (1946) who isolated 

 phosphocreatine in the form of a calcium salt, from the testes of the 

 carp. 



ADRENALINE AND NORADRENALINE 



Occurrence in semen and accessory organs 



Using 66% ethanol for the extraction of various tissues v. Euler 

 found that a substance closely resembling adrenaline is present in a 

 particularly high concentration in the prostate gland of man, dog, 

 rabbit and guinea-pig, in the seminal vesicle of bull and ram, and 

 in the ampulla ductus deferentis of dog, bull and ram; the amount 

 of the active substance corresponded to 1-5 //g. of adrenaline per 

 g. of fresh tissue. 



Results obtained by Brochart (1948«) with the colorimetric method 

 strengthened the view that adrenaline occurs as a normal consti- 

 tuent in the semen of bull (1 /<g./ml.), goat (l-5-l-7/ig./ml.) and man 

 (10-21 /ig./ml.); but later, Beauvallet and Brochart (1949) came to 

 the conclusion that in the bull at any rate, the pressor activity of 

 semen is due partly to adrenaline, and partly to noradrenaline. 

 H H 



I I 



HO— C C CHaCH., HO— C C CH^CHg 



II I II II I I I 



HO— C C— H OH NH CHg HO— C C— H OH NHg 



I I 



H H 



Adrenaline (epinephrine) Noradrenaline 



Enzymic oxidation 



When adrenaline or noradrenaline are added to bull semen in 

 relatively high concentrations (10-100 /^g./ml.), the aerobic but 



