6] ELECTROPHORESIS AND CHROMATOGRAPHY 99 



essentially to the separation and if each component can be eluted at a bufTer 

 concentration giving /?/=!, mutual displacement effects may contribute very 

 much to the separation of closely similar substances, and in this case a com- 

 paratively long column is of value. Such displacement effects are very 

 common and often very pronounced among proteins. Zones may often be 

 found which during migration through the column show marked separa- 

 tion without splitting up into two distinct peaks, the slower material being 

 carried along with the faster by the reduction in affinity to the column caused 

 by the displacement effect. It appears likely that a closer study of such dis- 

 placement effects may lead to a better understanding of the phenomena 

 underlying protein chromatography and perhaps also to more efficient 

 and rational modes of operation. 



In the discussion to follow, Dr H. Boman will show some results which 

 illustrate the importance of displacement effects in the chromatography of 

 some proteins. 



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