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MUTUAL DISPLACEMENT IN CHROMATOGRAPHY 101 



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Fig. 1. (a) Upper part: One-step elution of 100 mg. horse-radish peroxidase and acid 

 phosphatase on a 180 ml. column of Dowex 2. Fraction volumes about 0-65 ml. For 

 details see ref. 3. (6) Lower part: One-step elution of 35 mg. horse-radish peroxidase and 

 acid phosphatase on a 17 ml. column of TEAE cellulose. Fraction volumes 0-5-0-6 ml. 

 For details see ref. 4. 



centration has been kept constant through each experiment and the de- 

 veloper used was a aa-globuHn fraction from human serum (kindly supplied 

 by AB KABI, Stockholm). The results from three experiments carried out 

 at different buffer concentrations are given in Fig. 2. At the start, the column 

 was in equilibrium with a THAM (2-amino-2-hydroxymethylpropane-l : 3 

 diol)-HCl buffer of pH 7-3. In each experiment, the same amount of freeze- 



