102 HANS G. BOMAN [6 



dried prostatic extract (1 mg.) was dissolved in the corresponding buffer 

 and applied to the column. Elution was then directly started with a 0-1% 

 solution of aa-globulin, also dissolved in the corresponding buffer. Phos- 

 phatase activity was determined and expressed as described earlier.^ 

 The upper part of Fig. 2 shows that with 0-02m buffer, only small amounts 



02M THAN -HCl 



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40 Tube number 



10 



OOeM THAM-HCI 



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40 Tube number 



OIOM THAM-HCI 



40 Tube number 



Fig. 2. Displacement analysis of prostatic acid phosphatase at three different concentra- 

 tions of THAM-HCI buffers of pH 7-3. In each experiment, 1 mg. of prostatic extract 

 was applied to a column with 20 ml. of Dowex 2 and developed with a 0*1% solution of 

 og-globulin from human serum. Fraction volumes around 0-8 ml. 



of phosphatase (recovery 4%) are eluted as a very broad zone and that the 

 front of the activity coincides with that of the developer. As a whole, this 

 experiment looks like an unsuccessful frontal analysis. However, when the 

 adsorption of the phosphatase is decreased by increasing the buffer con- 

 centration to 0-06m, 22% of the activity appears as a localized zone, just at 

 the beginning of the front of the developing aa-globulin (Fig. 2, middle 

 part). With a further decrease in the adsorption of the enzyme, when using 

 a 0-IOm THAM-HCI buffer, 62% of the activity is recovered in a sharp 

 zone which has moved forward with regard to the front of the developer 

 (Fig. 2, lower part). The small peak around tube 10 is non-adsorbed material. 



