no L. C. CRAIG, W. KÖNIGSBERG, A. STRACHER, T. P. KING 

 be separated by dialysis at 65° even though their molecular weights are very 

 similar. 



Table 3 

 EFFECT OF pH ON 50% ESCAPE TIME 



Table 3 shows the effect of pH at 25°. The results with bacitracin were 

 obtained with 18/32 Visking, but 20/32 Visking was used for all the others. 

 Bacitracin is known to be unstable below a pH of 2 and above a pH of 7. 

 Ribonuclease shows the behavior to be expected and is quite different from 

 oxidized ribonuclease. Lysozyme shows a markedly slowed rate of dialysis 

 only at the pH extremes. Autodigestion probably plays a role with trypsin 

 and chymotrypsin at the higher pH. 



Table 4 

 DIALYSIS IN UREA SOLUTION 



* Done in OlN acetic acid. 



