7] DIALYSIS STUDIES 115 



polypeptides and proteins indicates that both the molecular weight and the 

 shape must be considered. 



If the molecular weight is known then dialysis can be a useful tool for 

 studying the shape of the molecule in a particular solution. Something about 

 the change in shape brought about by a change in environment can be re- 

 vealed. Information about the state of aggregation also can be derived. 

 Conversely, if the shape of the molecule is known a good estimate of the 

 molecular weight can be made. 



Finally, dialysis as outlined here can be a very useful separation tool 

 which at the same time offers a degree of characterization with respect to 

 size homogeneity. 



BIBLIOGRAPHY 



1. R. SIGNER, H. HANNI, W. KOESTLER, W. ROTTENBERG and P. VON TAVEL, 



Helv. Chim. Acta, 29. 1984 (1946). 



2. J. H. NORTHROP and M. L, ANSON, /. Gen. Physiol., 12, 543 (1929). 



3. L. c. CRAIG and t. p. king,/. Am. Chem. Soc, 11, 6620 (1955). 



4. R. E. STAUFFER, in Weissbcrger, Technique of Organic Chemistry, Vol. Ill, 2nd 



edition, p. 79, Interscience Publishers, New York, 1956. 



5. L. c. CRAIG, T. p. KING and A. STRACHER, /. Am. Chem. Soc; 79, 3729 



(1957). 



6. w. HAUSMANN, J. Am. Chem. Soc, 78, 3663 (1956). 



7. s. MOORE and w. h. stein,/. Biol. Chem., 211, 907 (1954). 



8. h. neurath, j. a. rupley and wm. j. dkeyur. Arch. Biochem. Biophys., 65, 



243 (1956). 



9. E. neurath and wm. j. dreyer. Dis. Faraday Soc, No. 20, 32 (1955). 



10. WM. F. HARRINGTON and J. A. SCHELLMAN, Compt. rend. trav. lab. Carlsberg, 

 30, 21 (1956). 



11. E. J. HARFENIST and L. c. CRAIG, /. Am. Chem. Soc, 74, 3087 (1952). 



12. D. F. WAUGH, in Ciba Foundation Colloquia on Endocrinology, Vol. 9, p. 122, 



Internal Secretions of the Pancreas, J. and A. Churchill Ltd., London, 1956. 



13. c. H. w. HiRS, s. MOORE and w. H. STEIN, /. Biol. Chem., 200, 493 (1953). 



14. J. R. PAPPENEIMER, Physiol Rev., 33, 387 (1953). 



15. H. SPANDAU, Angew. Chem., 63, 41 (1951). 



16. H. SPANDAU and e. brunneck, Angew. Chem., 65, 183 (1953). 



