0.6- 



^ 0.3 - 



1.0 



1.5 



2.0 2.5 



EFFLUENT VOLUME 



3.0 

 (LITRES) 



4.0 



Fig. 1. Chromatographic fractionation of bovine ribonuclease (Armour, 381-59) by the 

 method of Hirs, Moore and Stein (1953). Numbers refer to the contents of individual 

 tubes used in the experiments shown in Fig. 2. 'A' indicates the tubes pooled for the 

 determinations in Tables 1 and 2. 



Fig. 2. Drawing of precipitin zones apparent in agar after 3 days of development. Circles 

 denote the position of cups in the agar. S . . . . indicates the position of cups filled with 

 ribonuclease antiserum, t shows cups containing chromatographic fractions of ribonu- 

 clease (Armour, 381-59); numbers refer to tube numbers (Fig. 1). 



