324 



CHOH HAO LI 



pH 5.1, 0.057 M Nd*, 045 M (NH4)2S04 



[18 



25 50 75 



Tube Number 



100 



Fig. 11. Upper: Chromatography on a cation exchanger, Amberlite IRC-50 resin, of 

 whale somatotropin concentrate (0-2-0 -4 SAS fraction); 3 cm. diameter column contain- 

 ing 220 ml. pH 5-1 buffer applied to the column; 15-5 ml. /tube. 



Lower: Chromatography on a cation exchanger, Amberlite IRC-50 resin, of highly 

 purified whale somatotropin; 0-9 cm. diameter column containing 20 ml. resin; 15 mg. 

 protein in 10 ml. pH 5 1 buffer applied to the column; arrows indicate same sequence of 

 buffer as upper diagram; 3 ml./tube. 



Table 10 



ACTION OF CHYMOTRYPSIN ON 

 WHALE GROWTH HORMONE 



* pH 9-5, enzyme/substrate, 1/300 (w/w), 25°C. 



t A total dose of 40 micrograms in 4 days. 



% Mean ± standard error; tibia width for the controls, 160 /*. 



