. -4, .ip >y ^ .^ H^. ..., ,j ,~. ,,; .^ ,»( .^ ,; ^ 



# t # # « vS^- 1^ -^ # V -.«*■ r ■#%* ,rf* .',. 





Subject Index 



ACTH, see Corticotropin 

 Absorption spectra, ultra-violet 

 of myoglobin, 144, 147 

 of ribonuclease, 233-234, 236 

 of tobacco mosaic virus, 252-253 

 Acetyl groups in proteins, 259-260, 



335 

 Albumin, see Serum albumin 

 Amide group, configuration of, 18-21 

 localization in peptides, 192, 218- 

 219 

 Amino acids 

 desalting of, 257 

 quantitative estimation of, 



by paper chromatography, 91, 



116-121 

 by isotope dilution, 116-121 

 as DNP derivatives, 257 

 Amino groups, terminal, determina- 

 tion (see also Peptides, struc- 

 ture determination) 

 by dinitrofluorobenzene, 91, 264, 



267-268 

 by Edman degration, 215-218, 



264-266 

 by leucinaminopeptidase, 192,218- 

 219 

 Aminopeptidase, see Leucinamino- 

 peptidase 

 Antibodies 

 as enzyme inhibitors, 244-246 

 enzymic digestion of, 290 

 to growth hormones, 326 

 to ribonuclease, 241-246 

 to serum proteins, 298-301 

 to silk fibroin, 291 

 to tobacco mosaic virus, 262 

 Antigen-antibody reaction, 246, 293- 

 294 

 as criterion of homogeneity, 240- 



241 

 by diffusion in gels, 241-243, 292, 



299-301 

 inhibition of, 291-294 



Asparagine, localization in peptides, 

 192, 218-219 



Bacitracin, dialysis rate, 108-110 



Carbobenzoxylation of -NH^, groups, 



224-232 

 Carboxyl groups, terminal, determina- 

 tion (see also Peptides, struc- 

 ture determination) 

 by carboxypeptidase, 171, 218-219, 

 233, 250, 263-264, 287, 315, 

 318, 322 

 by hydrazinolysis, 171, 215, 218, 



258-260, 264, 310, 315, 317 

 by LiAlH4 reduction, 277, 330-332 

 Carboxypeptidase, action on 

 enolase, 338-340 

 growth hormone, 318, 322 

 lysozyme, 277, 285 

 poly-DL-alanine, 33 

 prolactin, 315, 317 

 ribonuclease, 233 

 tobacco mosaic virus, 250, 263-264 

 Carboxypeptidase B, action on tryp- 

 sin, 171 

 Chromatography of 

 amino-acids on paper, 91, 116-121 

 peptides 

 combined with paper electro- 

 phoresis, 148-150,257 

 on ion exchange resins, 161, 

 186-192, 212-221, 257, 281, 

 283, 285-286 

 proteins 



on calcium phospate, 96-99 



on cellulose derivatives, 100-103, 



338 

 on ion exchange resins, 100-103, 

 197, 199, 200, 324 

 Chymotrypsins, a, a^, 8, v, 155-167 

 action on 



asparaginyl bonds, 161-162, 175, 

 213 



