a^ # ^ 



4 ^ %r 



INDEX 



349 



Peptides — cont. 



fractionation by electrophoresis, 



78, 79, 90, 227-229, 268, 281 



ion-exchange chromatography, 



161, 186-192, 212-221, 257, 



281, 283, 285-286 



paper chromatography, 78, 79, 



90,268,281 

 partition chromatography, 268 

 two-dimensional electrophoresis 

 chromatography, 148-150, 257 

 structure determination, 148, 215- 

 229, 257-258, 281-282, 305- 

 312, 333-335 (see also Amino 

 groups, terminal and car- 

 boxyl groups, terminal) 

 Performic acid oxidation, 175, 186, 

 191, 211, 220, 224-232, 236, 

 282-284, 316, 322 

 Peroxidase, chromatography of, 100- 



103 

 pH and protein configuration, 45-60 

 Phenolic groups in proteins, see 



Tyrosine 

 Phosphatase, chromatography of, 100- 



103 

 Pituitary hormones, 302-327, 333-337 

 (see also under individual 

 hormones) 

 Poly-DL-alanine, 24-34 



action of peptidases on, 33-34 

 deuterium exchange in, 26-33 

 molecular species in solution, 31 

 physico-chemical properties, 25 

 Polymethacrylic acid, 47-49, 5 1 



Polypeptides, synthetic 



a-helix in, 17, 24, 33, 36 



hydrogen bonds in, 19-20, 23-34 

 Poly-DL-serine, deuterium exchange, 



33 

 Prolactin, 314-318, 330-332 



chemical modification and activity, 

 317 



oxidation, 315 



physico-chemical properties, 315 



purification, 314 



reduction, 316 



structure, partial, 317-318 

 Protamine, fractionation by dialysis, 

 107-108 



Proteins 



chemical modification with reten- 

 tion of activity 

 antibodies, 290 

 chymotrypsinogen, 159-163 

 corticotropin, 312, 335-336 

 growth hormone, 318-319, 322, 



324, 326 

 insulin, 290 

 papain, 193, 197-200 

 pepsin, 179-181 

 serum albumin, 290-294 

 tobacco mosaic virus, 251, 290 

 trypsinogen, 171 

 configuration, 25-62 

 homogeneity, 93-99, 140-141, 144, 

 183, 262-263, 304, 308, 314, 

 316, 323-325 

 immunological criteria, 1 84, 240- 

 244 

 purification, 

 chromatography, 96-1Û3, 197, 



199, 241-243, 324 

 countercurrent distribution, 304, 



314 

 electrophoresis, 94-97, 140-141, 

 291, 307-308, 316 

 species specificity, 66-75, 144-147, 



305-327 

 structure, genetic control of, 148- 



151, 247, 262-263, 298-301 

 surface, polarity of, 43 

 PyrroUdone carboxyl peptides, 268 



Random coils, in denatured proteins, 

 48-59 

 in polyelectrolytes, 35-38, 47-49 

 Ribonuclease 

 action of carboxypeptidase on, 233 

 chymotrypsin on, 212, 219-220 

 pepsin on, 233-235 

 subtilisin on, 229, 233, 236 

 trypsin on, 211-220 

 amino acid sequence, 213, 230 

 carbobenzoxylation, 224 

 dialysis rate, 108, 110 

 disulphide bonds, oxidation, 211, 

 219-221, 229, 236 

 reduction, 224-228, 230-232, 236 

 immunological heterogeneity, 241- 

 246 



