CONTENTS 



Page 



Preface v 



PART I 



Culture Media 



Principles of Microbial Nutrition and Composition of Culture Media 



for Microorganisms 1 



General Directions for the Preparation of Culture Media 3 



General Media for Isolation and Cultivation of Bacteria 8 



Media for Anaerobic Spore-forming Bacteria 11 



Media for Fungi 12 



Media for Yeasts 16 



Media for Actinomyces 18 



Media for Protozoa 19 



Media for Algae 20 



Media for Urea Bacteria 21 



Media for Nitrifying Bacteria 22 



Media for Nitrate-reducing and Denitrifying Bacteria 25 



Media for Reduction of Sulphates and Other Sulphur Compounds . . 26 



Media for Sulphur-oxidizing Bacteria 28 



Media for Hydrogen and Methane Bacteria 30 



Media for Iron and Manganese Oxidizing Bacteria • . . . . 31 



Media for Nitrogen- fixing Bacteria 32 



Media for Cellulose-decomposing Bacteria 35 



Special Media 39 



Preserving Stock Cultures 45 



Favorable Conditions for the Development of Anaerobic Bacteria. . . 46 



PART II 



Methods of Staining 



Methods of Staining Bacteria 47 



PART III 



Qualitative and Quantitative Methods of Analysis 



Preparation of Reagents 52 



Preparation of Standard Solutions 54 



Qualitative Methods for Determining Various Forms of Inorganic 



Nitrogen 56 



Determination of Moisture in Soil 61 



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