CULTURE MEDIA 11 



Medium 9 



ASPARAGIN MaNNITOL AgAR^ 



Agar 15.0 gm. 



Dipotassium phosphate (K2HPO4) 1.0 gm. 



Potassium nitrate (KNO3) 0.5 gm. 



Magnesium sulphate (MgS04-7H20) 0.2 gm. 



Calcium chloride (CaGl2) 0.1 gm. 



Sodium chloride (NaCl) 0.1 gm. 



Ferric chloride (FeCl3-6H20) trace 



Asparagin (C4H8N2O3) 0.5 gm. 



Mannitol 1.0 gra. 



Water 1,000.0 cc. 



1 Thornton, H. G., Ann. Appl Biol, 9: 241-274, 1922. 



After the agar and salts have been dissolved add the mannitol, 

 adjust the reaction to pH 7.4 (Brom thymol blue) and filter. 

 Mannitol is recommended in place of glucose or related sugars 

 because it decomposes less than the common sugars during 

 sterilization. 



Medium 10 

 Tap- WATER Gelatin 2 



Gelatin 120 to 200.0 gm. 



Tap water 1,000.0 cc. 



2 Conn, H. J., N. Y. Agr. Expt. Sta., Tech. Bull 57, 1917. 



Reaction pH 6.5. 



Incubate the plates at 18°C. for 7 days. This medium is 

 especially useful in qualitative studies of soil flora. 



MEDIA FOR ANAEROBIC SPORE-FORMING BACTERIA 



Medium 11 



Glucose-phosphate Nitrogen Free Medium for Anaerobic Bacteria^ 



Dipotassium phosphate (K2HPO4) 1.0 gm. 



Magnesium sulphate (MgS04-7H20) 0.2 gm. 



Sodium chloride (NaCl) 0.01 gm. 



Ferrous sulphate (FeS04-7H.20) 0.01 gm. 



Manganese sulphate (MnS04-4H20) 0.01 gm. 



Glucose 20.0 gm. 



Calcium carbonate (CaCOa) 30 . gm. 



Distilled water 1,000.0 cc. 



3 Winogradsky, S., Centr. BakL, II Abt., 9: 49, 1902. 



